Animal's castration vaccine gene and gene engineering body


Application Number: 00107831
Application Date: 2000.06.23
Publication Number: 1277261
Publication Date: 2000.12.20
Priority Information:
International: A61K39/395;C12N15/13;C12N15/63;C12N15/64;C12N15/70
Applicant(s) Name: Nanjing Agricultural Univ.
Address:
Inventor(s) Name: Ji Chuanyi;Du Nianxing;Zhu Jieqing
Patent Agency Code: 11002
Patent Agent: qian baoyang
Abstract The present invention is used in producing castration gene engineering subunit vaccine for farm animal and relates to the field of biological hi-tech. The technological process includes artificial synthesis of LHRH gene segment for farm animal with Ncol and Xhol enzyme incision sites separately in its 5' and 3' end, insertion of pET-32c plasmid to constitute four kinds of recombined pET-32cLHRH-N/X plasmid expressing LHRH-Trx fusion protein, and conversion of recombined plasmid into colon bacillus BL21(DES). The expression product may be soluble protein easy to purify, and is used universally for livestock and poultry to castrate effectively.