Establishment of anti-morphine single anti-cell strain and method for producing double-side morphine test paper


Application Number: 00109054
Application Date: 2000.06.02
Publication Number: 1327158
Publication Date: 2001.12.19
Priority Information:
International: G01N33/52;G01N33/53;G01N33/531;G01N33/577
Applicant(s) Name: Beijing Univ.
Address:
Inventor(s) Name: Wang Nengdong;Chen Jiahua;Zhang Xiu
Patent Agency Code: 11241
Patent Agent: zhang huahui
Abstract The present invention relates to the production of double-side immunological chromatographic test paper for simultaneous detection of free morphine and total morphine in urine sample, It includes the steps of the preparation of N place, 3 place and 6 place morphine-protein compound; establishment of anti-morphine monoclonal antibody cell strain and the preparation of anti-morphone monoclonal antibody and polyclonal antibody; the preparation of immunological compatible chromatographic fixed phase; the immunizing compatible pure monoclonal antibody and polyclonal antibody, and cross purifying polyclonal antibody; the preparation of anti-morphine composite; the preparation of chromatographic film for the immunological chromatographic test paper; and assembly of the test paper.