Method for amplifying and labeling nucleic acid moleculae


Application Number  00129324 Application Date  2000.11.15
Publication Number  1353199 Publication Date  2002.06.12
Priority Information    
International
Classification
 C12P19/34;C12Q1/68  
Applicant(s) Name  Wu Chang  
Address    
Inventor(s) Name  Wu Chang;Wu Ming  
Patent Agency Code  00000 Patent Agent  
AbstractA process for amplifying and labelling nucleic acid molecules includes polymerase china reaction and transcription reaction, and features that before polymerase chain reaction, the 3' terminal of DNA is extended by terminal transferase, the primer of epicole promoter complementary with the said extended sequence and the primer specific to 5' terminal are used for PCR amplfication, and the RNA polymerase is used to amplifying the said PCR amplfied DNA product to obtain the labelled RNA. Its advantages is low cost.