Method for creating transgenic receptor system of corn and application of same

Application Number: 00110842
Application Date: 2000.01.26
Publication Number: 1263949
Publication Date: 2000.08.23
Priority Information:
International: A01H4/00;C12N5/04;C12N15/87
Applicant(s) Name: Shandong Univ.
Inventor(s) Name: Zhang Juren;Li Xuehong;Quan Ruidang
Patent Agency Code: 37200
Patent Agent: sun jun
Abstract The present invention discloses a method for creating transgenic receptor system of corn and its application. The stem tip of corn is inoculated to the inducing culture medium added with combined hormones to induce the cultured stem tip to directly grow tussock uds or tissue block of tussock bud. Its next generation is cultured and its tussock buds or the tissue block of tussock bud are used as receptor. After the exogenous genes are transferred into cultured cells, such steps as recovery and screening are used to obtain transgenic plants. Its advantages are use of the most of gene-type cultured cells to regenerate transgenic plant, high frequency and less variation of characteristics.