Method for culturing fibrous root of tabacco to prepare nicotin

Application Number: 00107949
Application Date: 2000.06.01
Publication Number: 1276422
Publication Date: 2000.12.13
Priority Information:
International: A01H4/00;C12N5/04;C12P17/12
Applicant(s) Name: Research and Service Center of Nuisanceless Agricultural Chemicals, Xibei Agricu
Inventor(s) Name: Cao Yang;Gong Pubian;Dong Jianxin
Patent Agency Code: 11002
Patent Agent: lin xiangmeng
Abstract A process for preparing nicotin by cultivating fibrous root of tobacco includes such steps as infecting the explant of aseptic tobacco seedling with Agrobacterium of fibrous root, inducing the explant to grow fibrous root, detecting the T-DNA in root system using Southern bybridism to determine fibrous root line, cultivating the root system having T-DNA, immobilized continuous cultivating in modified MSO culture medium to obtain optimal fibrous root, and extracting nicotin fron the fibrous root.