Method for simultaneously extracting pathogen RNA and DNA in body fluid and special extracting liquid thereof


Application Number: 00114995
Application Date: 2000.03.20
Publication Number: 1314492
Publication Date: 2001.09.26
Priority Information:
International: C12N5/10;C12Q1/68
Applicant(s) Name: Bodan Gene Development Co., Ltd., Shanghai
Address:
Inventor(s) Name: Mao Yumin;Xie Yi;Xiao Zili
Patent Agency Code: 00000
Patent Agent:
Abstract The simultaneous extraction process includes the steps of: taking at least 5 micronliter for body fluid and adding 1-100 micronliter extracting liquid at 25-45 deg.c for 3-15 min; builing 5-15 min; maintaining at 50-80 deg.c for 10-30 min before uncovering; adding 2-10 micronliter of superpure water for suspension and resting for 5-20 min; centrifugalizing at 12,000-15,000 rpm for 5-15 min; and final preversing supernatant liquor. The special extracting liquid contains diethyl pyrocarbonate (DEPC) and NP40. The said process is simple, fast, high in yield and stable.