Multiple normalizing gene amplification method


Application Number: 00110816
Application Date: 2000.01.10
Publication Number: 1266104
Publication Date: 2000.09.13
Priority Information:
International: C12Q1/68
Applicant(s) Name: Shandong Provincial Pharmaceutical Biological Tech. Research Center
Address:
Inventor(s) Name: Han Jinxiang
Patent Agency Code: 37200
Patent Agent: sun jun
Abstract the present invention relates to a multiple normalized gene amplification method, and is characterized by that the addition of consensus primer fragment whose length is 17-25 nucleotides at 51 end of amplicon is adopted, through primary specific amplification, the consensus primer is used to trigger amplification, and finally the amplification of consensus primer is normalized. Said invented method can amplify more than 30 genes simultaneously, so greatly raises the working efficiency of gene analysis.