Process for detecting gene mutation on glass plate

Application Number: 00116095
Application Date: 2000.10.25
Publication Number: 1293255
Publication Date: 2001.05.02
Priority Information:
International: C12Q1/68
Applicant(s) Name: Wuhan Inst of Viruses, Chinese Academy of Sciences
Inventor(s) Name: Zhang Xianen;Wen Jikai;Deng Jiaoyu
Patent Agency Code: 42001
Patent Agent: wang minfeng
Abstract A method for detecting gene mutation on glass plate includes such steps as fixing the hybridized complete DNA heteroduplex to glass plate to make the 5' terminal of one of said double strands be cross-linked with mercapto silanized glass, modifying the cytosine in mismatched base with hydroxylamine solution and then with mixed solution of potassium permanganate and tetraethylamine chloride, shearing the modified pyrimidine base with piperidine, digesting the DNA heteroduplex wth (2-5)-unit SI acid enzyme, and mutation detection by linking human colibacillus basic phosphorylase. Its advantages are high correctness, sensitivity and speed, and low cost.