Recombination human epidermal growth factor gene, its expression product and its application


Application Number: 00117272
Application Date: 2000.07.19
Publication Number: 1279288
Publication Date: 2001.01.10
Priority Information:
International: A61K38/18;C07K14/485;C12N1/21;C12N15/12;C12N15/70
Applicant(s) Name: Huashengyuan Gene Enginering Development Co., Ltd., Shenzhen
Address:
Inventor(s) Name: Tu Guihong;Li Huangjin;Shi Yongfeng
Patent Agency Code: 44200
Patent Agent: lin canzhi
Abstract A recombined human epidermal growth factor (rhEGF) gene is synthesized on the basis of colibacillus prefered codens. The carrier containing said gene and recombination strawn are constructed. Said gene contained colibacillus expression carrier pT48, its transformed recombined colibacillus strawn JM109CDE30/pT48 and the process for preparing rhEGF from said strawn are disclosed. Said rh EGF features high expression quantity and efficiency and easy being purified. The rhEGF can be used to prepare medicinal spray to treat skin wound with high curative effect.