Recombined anti-human TNF alpha gene engineering anti-bogy and its preparing method


Application Number: 00113874
Application Date: 2000.07.14
Publication Number: 1280190
Publication Date: 2001.01.17
Priority Information:
International: C12N15/13;C12N15/63
Applicant(s) Name: No.4 Military Medical Univ., PLA
Address:
Inventor(s) Name: Chen Ping;Deng Jianpei;Han Hua
Patent Agency Code: 61110
Patent Agent: zhao weiyong
Abstract Of the anti-human TNF alpha engineering antibody, the gene sequence has a record number of AF252545 and AF 262753 in Genebank. The preparation process includes the main steps of: reverse transcription polymerase chain reaction to obtain the whole sequence and partial sequence of required anti-human TNF alpha gene engineering antibody; gene engineering measure to obtain the recombined expressed product of anti-human TNF alpha gene engineering antibody; and purification of the product to obtain recombined anti-human TNF alpha gene engineering antibody with high activity. The antibody of present invention has the functions of neutralizing TNF alpha toxicity and protecting tissue.