Single-basic extension signal discrimination method


Application Number  00129344 Application Date  2000.11.22
Publication Number  1354261 Publication Date  2002.06.19
Priority Information    
International
Classification
 C12Q1/68  
Applicant(s) Name  Wu Chang  
Address    
Inventor(s) Name  Wu Chang;Wu Ming  
Patent Agency Code  00000 Patent Agent  
AbstractThe present invention relates to a monobase elongation signal discrimination method. Including hybridization of target molecular and SBE primer fixed on the solid surface, making SBE reactino and analyzing signal collected after SBE reaction. It is characterized by that the described target molecular possesses a labeled molecular which can be identified, and the dideoxyribonucleotide used by SBE reaction processes other labeled molecule, and the proportion of the signal obtained by SBE reaction to the labeled molecule signal in target molecular in same position can be calculated so as to determine that said reaction signal is true or false. Said invention eliminates the false positive reaction, and does not produce misiagnosis.