Twin introducer used for homogeneous fluorescent PCR testing

Application Number: 00108413
Application Date: 2000.04.30
Publication Number: 1278009
Publication Date: 2000.12.27
Priority Information:
International: C12Q1/68
Applicant(s) Name: Xiamen Univ.
Inventor(s) Name: Li Qingge;Guo Qiuping;Luan Guoyan
Patent Agency Code: 35200
Patent Agent: chen yongxiu
Abstract The present invention relates to double stranded primer capable of preventing non-specific amplification and used in homogeneous fluorescence PCR detection-twin primer. The twin primer is obtained from hybridization of positive primer and negative primer, the positive primer is upper and lower stream primers used for amplification, negative primer is the sequence compensated and modified with positive primer including labelled type and non-labelled types. It is made to lose extendobility by labelling or non-labelling on 5' end of posetive primer and modifying on 3' end of negative primer. The double stranded twin primer can effectively prevent non-specific amplification in PCR process, it is simple in preparation and can be applied to different PCR detection technique flexibly.