Protein chip, its preparing process and its application in screening monoclonal antibody


Application Number: 00105820
Application Date: 2000.04.13
Publication Number: 1274085
Publication Date: 2000.11.22
Priority Information:
International: G01N33/68
Applicant(s) Name: Chen Xueyin
Address:
Inventor(s) Name: Chen Gaoming
Patent Agency Code: 11138
Patent Agent: yao baoqin
Abstract The present invention relates to a protein chip, its preparing process and a sensitive method using it to screen monoclonal antibody. It includes immunizing BALB/C mouse with uniform tissue slurry, fusing the kidney cells with myeloma cells of BALB/C mouse, amplification culture, preparing protein chip while creating hybridoma cells library, preparing protein for creening, and using specific antigen and polyclonal antibody to screen relative monoclonal antibody. It can be used for large-scale quick screening of monoclonal antibodies.

Category: Uncategorized