Short-chain neurotoxin of sea serpent and gene for coding it


Application Number: 00124831
Application Date: 2000.09.18
Publication Number: 1343689
Publication Date: 2002.04.10
Priority Information:
International: C07K14/435;A61K38/17;A61P25/00
Applicant(s) Name: Zhongshan Univ
Address:
Inventor(s) Name: Xu Anlong;Zhong Xiaofen;Peng Lisheng
Patent Agency Code: 11021
Patent Agent: hu jiaoyu
Abstract By configuring the cDNA library for poison gland of lapemis hardwicki and sequencing its DNA, three genes sn12, sn36 and sn160 for coding short-chain neurotoxin are obtained, which are cloned to expression carrier PETTRX by PCR method to modify and to amplity them. The BL21-DE3 is used as expression host. Through IPTG induction, they have the soluble efficient fusion expression in colibacillus. The said genes can greatly increase the pain threshold.